Amplification-based detection methods at DNA level such as PCR and isothermal alternatives such as RPA are sensitive, specific and can be established quickly, but are susceptible by the carryover of amplicons from previous reactions. PCR also requires a precise sequence of different temperature steps and therefore an appropriate equipment and trained personnel. Outside of a laboratory environment, these two methods are therefore almost impossible to use.
By using a further sequence-specific oligonucleotide in RPA reactions, this project is now developing highly sensitive, robust and easy-to-use rapid tests which, driven by body temperature, enable a broad application without equipment or specialist personnel and provide a reliable result even in the presence of amplicons from previous tests.
In cooperation with an industrial partner, a rapid test for a pathogen relevant to the agricultural sector is currently being implemented.